Single-molecule analysis for molecular haplotyping
نویسندگان
چکیده
منابع مشابه
Single-molecule dilution and multiple displacement amplification for molecular haplotyping.
Separate haploid analysis is frequently required for heterozygous genotyping to resolve phase ambiguity or confirm allelic sequence. We demonstrate a technique of single-molecule dilution followed by multiple strand displacement amplification to haplotype polymorphic alleles. Dilution of DNA to haploid equivalency, or a single molecule, is a simple method for separating di-allelic DNA. Strand d...
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Several million SNPs have been identified and are available in the public databases. For most, no effect on gene function or expression is known. “Functional polymorphisms” refers to SNPs to which a putative effect on gene function or expression is attributed and implies that they are “non-silent” variations located in regions that are coding, regulatory, splice sites, or untranslated regions t...
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ژورنال
عنوان ژورنال: Human Mutation
سال: 2004
ISSN: 1059-7794,1098-1004
DOI: 10.1002/humu.20020